Determination of Aflatoxin M1 and B1 in Egyptian Raw Milk, Soft Cheese and Table Eggs using ELISA Technique

This study was conducted to determine the AFM1 levels in Egyptian raw milk, soft cheese and AFB1 in table eggs marketed in Kafrelsheikh city markets. Aflatoxin content was measured using microtiter-plate enzyme-linked immunosorbent assay (ELISA). The Test Kits, RIDASCREEN ELISA of Biopharm AG that used is based on competitive enzyme immunoassay for the quantitative analysis of Aflatoxin AFM1 and AFB1 and with detection limit of 5ppt for AFM1 and 1ppb for AFB1. Tests and standards were performed according the instruction of manufacturer and the results were measured on standard curve of concentrations ranged from 5 to 80 ppt for AFM1 and from 1000 to 2000 ppt for AFB1. Of 90 milk samples, 86 (95.6%) samples contained aflatoxin M1 with concentrations ranged from 5.3 to 43.5 ppt and the mean value was 10.4 ppt. 46 cheese samples (92%) contained AFM1 with concentrations ranged from 50 to 97 ppb and average value of 71 ppb while 2 (4%) of table eggs examined contained AFB1 with quantities ranged from 1100 to 1700 ppt. Data also indicated that AFM1 residues concentrations detected in all the positive samples were above the permitted levels of ESO and below the tolerated levels foreign standards. So it could be concluded that contamination of AFM1 in milk and cheese marketed in Kafrelsheikh city appear to be of serious public health problem at the moment while table eggs needs more further examination at large scale.